How much media should i load?

With the ICE Vitrification system, the amount of media that should be loaded into the straw does not matter. You can load a large column with your cells or a small column. The cooling rate is not important, therefore a large or small volume will work.

We suggest you load a 1-2cm column of media with your cells, or approximately 20-40ul. For oocytes we suggest a slightly smaller column of 10ul of media.

The media and cells will vitrify in either a large amount or small amount of media. The cooling rate will be slightly greater with a tiny amount of media but that does not affect survival in this system. The reason is that the media is very different than other systems.